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Description

Apply a light coating of olive oil to the suppository before insertion

Sugano K, Maeda K, Ohtani H, Nagahara H, Shibutani M, Hirakawa K

2006;13(15):1749-55. [PMID: 16787218]

Briefly, cFSP1 N eluted from the Ni-chelating resin was diluted with 0.1 X PBS buffer supplemented with 5% (v/v) glycerol and 5 mM -mercaptoethanol and loaded onto a HiTrap Heparin HP column (GE Healthcare, USA)